Description
Omega Biotek E.Z.N.A.® Fungal RNA Mini Kit
Isolate total RNA from fungal samples using spin columns
Modified from the E.Z.N.A.® Plant RNA Kit, the E.Z.N.A.® Fungal RNA Kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, Northern Blotting, Poly A +(mRNA) purification, and differential display.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-100 μL |
| Starting Material | Fungal Tissue |
| Starting Amount | <250 mg |
| RNA Yield | Up to 100 µg, >200 nt |
| Processing Mode | Spin column |
| Throughput | 1 – 24 |
| RNA Binding Technology | 1 – 24 |
| Binding Capacity | 100 µg |
