Description
Omega Biotek E.Z.N.A.® FFPE RNA Kit
Isolates RNA from the FFPE samples using spin columns
The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNase’s and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, micro-array, Northern blot, poly-A purification |
| Elution Volume | 15-30 μL |
| Starting Material | FFPE tissue |
| Starting Amount | 3-4 sections with a thickness of 10 μm |
| RNA Yield | Up to 50 µg |
| Processing Mode | Manual, centrifugation |
| Throughput | 1 – 24 |
| RNA Binding Technology | Silica Micro spin column |
| Binding Capacity | 50 µg |
